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Bilberry Extract Analytical Specification

Appearance Dark reddish violet amorphous hygroscopic powder
Solubility Soluble in methanol, very soluble in dehydrated alcohol and Practically insoluble in water
Identification By TLC The sequence of zones present in the chromatogram should match with obtained with the test solution and the test solution. Furthermore, other faint zones may be present in the chromatogram obtained with the test solution.
HPLC-Retention time The retention time of the major peak obtained with sample solution shall correspond to that of the major obtained in the Standard solution.
Loss on drying (%) (at 1050c for 3 hours) NMT 4.5
Total ash (%) NMT 2.0
Total Anthocyanidines (%) (as cyanidine chloride) NMT1.0
Assay (% on dried basis) cyanidine 3-o-glucoside chloride 32.4 to 39.6
Microbial parameters Total Bacterial Count Total Fungal Count Pathogens E.coli Salmonella P.aeruginosa S.aureus NMT 1000 cfu/g NMT 100 cfu/g Should be absent Should be absent Should be absent Should be absent